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KMID : 1177720200140010005
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Journal of Alternatives to Animal Experiments
2020 Volume.14 No. 1 p.5 ~ p.14
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Comparison Study of Antioxidant and Anti-inflammatory Effects for Raw or Fermented Lotus (Nelumbo nucifera) Leaf Extracts
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Kim Ji-Eun
Kim Bae-Hwan
Kwon Soo-Kyung
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Abstract
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This study tended to identify the potential as functional natural materials for soothing of skin and scalp using lotus leaf hot water extract (Nelumbo nucifera hot water extract, NHWE) and fermented lotus leaf extract (fermented nelumbo nucifera leaf extract, FNLE). The antioxidant evaluations such as total polyphenol and flavonoid content, DPPH electron-donating ability, and ABTS radical scavenging ability, were measured. Cell survival rate was measured in Raw 264.7 cells treated with LPS to check the toxicity, and the inhibitory rate of NO-producing and TNF-α and COX-2 protein expression were measured in Raw 264.7 cells after treatment of LPS in order to evaluate the efficacy of anti-inflammatory effect. NHWE and FNLE showed 182.2 mg/g and 103.3 mg/g each in total polyphenol contents, and 26.8 mg/g and 49.1 mg/g each in flavonoid content. As for ABTS radical scavenging ability, high levels of antioxidants (86.6% and 93.7% in NHWE and FNLE) appeared at the concentration of 1000 μg/mL. In DPPH electron-donating ability, NHWE and FNLE showed 66.1% and 73.3% at 1000 μg/mL. FNLE had more antioxidant ability than NHWE. In anti-inflammatory effects, inhibition rate of NO production by LPS increased in a concentration-dependent manner at both NHWE and FNLE, and TNF-α and COX-2 expressions were also decreased by treatment of NHWE and FNLE. In particular, 200 μg/mL of FNLE showed the best anti-inflammatory effect. In summary, fermented lotus leaf extract showed good efficacy in antioxidant and anti-inflammatory tests, and its potential as a functional ingredients was confirmed. It is expected that not only the soothing effect of the scalp and skin, but also the possibility of application as a functional food can be expanded, and it is considered to be suitable as a natural functional raw material.
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KEYWORD
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Nelumbo nucifera, antioxidant, anti-inflammatory, TNF-α, COX-2
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